Osmotic shock method was used to isolate the retinal vessels of rats; 采用渗透休克法获取大鼠视网膜血管。
Effects of osmotic shock on the uptake of ions by excised wheat roots 渗透震动对小麦离体根离子吸收的影响
Penicillin acylase was extracted by osmotic shock method. The specific activity of crude extract was 8.2 × 10-8 mol/ ( s · mg), it was ten-times higher than that from ultrasonic method. The total recovery was 93.3%. 采用渗透压冲击法提取青霉素酰化酶,粗酶液比活达8.20×10-8mol/(s·mg),比超声波法高10倍以上,酶活总收率达93.3%。
The activity yield of bead-vortexing, sonication and osmotic shock were 99.7%, 78.4% and 60.7% respectively while the others were below 22%. 结果表明玻珠震碎法、超声波法和渗透压法是较优的细胞破碎方法,活力回收率分别为99.7%、78.4%、60.7%,其他方法均低于22%。
Penicillin Acylase Extraction by Osmotic Shock Method 用渗透压冲击法提取青霉素酰化酶
However, high concentration of cryoprotective agents ( CPAs) necessary for vitrification can cause an osmotic shock and toxicity to cells. 但玻璃化法采用的高浓度玻璃化溶液不可避免地会造成化学毒性损伤和渗透压损伤。